中央大學系統生物與生物資訊研究所
Institute of Systems Biology and Bioinformatics
National Central University

CS_Chen 陳健生 (Chien-Sheng Chen)
副教授
美國康乃爾大學
研究室: 高通量生物感測實驗室 | 科學五館504室 | ext 36105
辦公室: 科學五館505室 | 電話: (03)4227151 ext 36103
E-mail: cchen103@gmail.com

專長領域 (Area of Competence)

蛋白質體微陣列晶片(Proteome microarray)、宿主-微生物相互作用(Host-microbile interaction)、奈米生醫感測科技(Nano-biosensing technology)


研究方向

蛋白質體學

利用蛋白質體微陣列晶片進行蛋白質體相關研究,特別是蛋白質體的交互作用。本實驗室除了具有大腸桿菌 (~4000 proteins) 蛋白質體微陣列晶片外,也具有酵母菌 (~5000 proteins) 和人類蛋白質體(~18000 proteins) 微陣列晶片。
Our lab has a new high-throughput protein purification technique to purify ~4000 E. coli K12 proteins within ten hours. The purified proteins were then spotted on glass slides to form an E. coli K12 proteome chip. It was successfully applied to many projects. First, new DNA damage recognition activities in Escherichia coli were identified. It was also showed an important DNA repair protein; ybaZ (ATL) binds abasic DNA strongly and this result was published in Nature Methods. In addition to DNA repair study, we also set out to perform human serum profiling for the identification of biomarkers in inflammatory bowel diseases and for characterizing the ontogeny of anti-E.coli antibodies using human sera derived from infants and adults. Part of the result was published in Molecular and Cellular Proteomics.

利用大腸桿菌蛋白質體微陣列晶片探討抗菌肽於細菌內部的作用機制,也用於探討大腸桿菌蛋白質體和人體的互動關係 (Host & Microbe Interaction)。
We attempted to find the intracellular target of natural antimicrobial peptides by our E. coliK12 proteome chips. Until now, we already discovered several intracellular proteins and verified them to ensure their importance for antimicrobial to inhibit cell growth. We also use bioinformatics analysis to understand the intracellular activity of antimicrobial peptides. Particularly, we elucidated the relationships between two response regulators belonging to two-component system (TCS) with Lactoferricin B. TCS is highly conserved among all the microorganisms and control the gene expressions for bacteria to grow under their unique niche environments. Our study showed that Lfcin B binds to two response regulators, BasR and CreB, of TCS. For further analysis, we conducted several in vitro and in vivo experiments and utilized bioinformatics methods. The electrophoretic mobility shift assays and kinase assays indicate that Lfcin B inhibits the phosphorylation of the response regulators (BasR and CreB) and their cognate sensor kinases (BasS and CreC). Antibacterial assays showed that Lfcin B reduced E. coli’s tolerance to environmental stimuli, such as excessive ferric ions and minimal medium conditions. This is the first study to show that an antimicrobial peptide attacks TCS directly. This study is in press by Molecular and Cellular Proteomics.
In addition to E. coli proteome chip, our lab also has yeast and human proteome microarrays. Thus, we are expending our research area from E. coli to yeast and human as well.

奈米生醫感測科技

應用奈米生化科技在多樣免疫分析技術 (simultaneous multiplexed immunoassays) 也是我們研究的重點。我們專長以蛋白質G-奈米脂粒 (protein G-liposomal nanovesicles) 以作為免疫分析的擴大訊號通用標劑 (universal reagent)。利用奈米脂粒,我們發展的感測科技平台為高通量的盤式系統和微陣列系統。所檢測的分子從小分子的抗生素到大分子的疼痛分子,甚至是細菌都是我們奈米生醫感測科技的檢測興趣範圍。

 


獲獎 (Awards and Honors)

2014  Distinguished professor, National Central University 2013  Academia Sinica Research Award for Junior Research Investigators
2013  Outstanding research award, National Central University
2008  Outstanding new faculty award, National Central University
2007  Poster Award of Protein Arrays, Protein Process Development, and Profiling Proteins at 2007 PepTalk conference
2005  Julian S. Colyer Award, Cornell University
2004  1st place, Graduate Scholarship Competition, Chinese American Food Society


研究室成員 (Members)

博士後研究員: 陳柏中 蕭士翔 許觀達
博七 宋子承 博四 楊安民 博一 Pramod Shah 碩二 林均穆
研究助理 KY Lu 陳怡彣


著作發表 (Publication)

書籍

  1. Chen C-S, Baeumner AJ, Durst RA (2008) Multiplexed immunoassays in food analysis. Handbook of Food Analysis Instruments Otles S (ed), CRC Press, Boca Raton, FL, p. 439-454.
  2. Chen C-S, Tao S-C, Zhu H (2008) Protein microarray technologies. Proteomics (Methods Express Series) O'Connor CD & Hames BD (eds), Scion Publishing Ltd., Bloxham, UK, p 183-205.
期刊 (2000~)

  1. Yeh S-B, Chen C-S*, Chen W-Y, Huang C-J. (2014) Modification of Silicone Elastomer with Zwitterionic Silane for Durable Antifouling Properties. Langmuir 16.
  2. Yi-Wen Chen, Ching-Hao Teng, Yu-Hsuan Ho, Tien Yu Jessica Ho, Wen-Chun Huang, Masayuki Hashimoto, I-Yuan Chiang, and Chien-Sheng Chen* (2014) Identification of bacterial factors involved in type 1 fimbria expression using an Escherichia coli K12 proteome chip. Molecular & Cellular Proteomics in press
  3. Sutandy FXR, F S-H Hsiao, Chen C-S* (2014) High throughput platform to explore RNA-protein interactomes. Critical Reviews in Biotechnology in press.
  4. Fan B, Lu K-Y, Sutandy FXR, Chen YW, Konan K, Zhu H, Kao CC*, Chen C-S* (2014) A human proteome microarray identifies that the heterogeneous nuclear ribonucleoprotein K (hnRNP K) recognizes the 5' terminal sequence of the hepatitis C virus RNA. Molecular & Cellular Proteomics in press.
  5. Ho T Y J, Chan C-C, Chan K, Wang Y C, Lin J-T, Chang C-M, Chen C-S* (2013) Development of a Novel Bead-based 96-well Filtration Plate Competitive Immunoassay for the Detection of Gentamycin. Biosensors & Bioelectronics 49:126-132.
  6. Sutandy F.X. R, Qian J, Chen C-S* and Zhu H* (2013) Overview of Protein Microarrays. Current Protocols in Protein Science 27.1.1-27.1.16.
  7. Su W-H, Ho T-Y, Tsou T-H, Lee W-L, Wang K-C, Yu Y-Y, Chen T-J, Tan C-H, Kuo C-D, Chen C-S*, Wang P-H* (2013) Development of a chip-based multiplexed immunoassay using liposomalnanovesicles and its application in the detection of pathogens causing femalelower genital tract infections. Taiwanese Journal of Obstetrics & Gynecology 52: 25-32.
  8. Guo S-L, Chen P-C, Chen M-S, Cheng Y-C, Lin J-M, Lee H-C, Chen C-S* (2012) A Fast Universal Immobilization of Immunoglobulin G at 4 °C for the Development of Array-based Immunoassays. PLoS ONE 7(12): e51370.
  9. Lu K-Yi, Tao S-C, Yang T-C, Ho Y-H, Lee C-H, Lin C-C, Juan H-F, Huang H-C, Yang C-Y, Chen M-S, Lin Y-Y, Lu J-Y, Zhu H, Chen C-S* (2012) Profiling lipid-protein interactions using non-quenched fluorescent liposomal nanovesicles and proteome microarrays. Molecular & Cellular Proteomics 11(11):1177-1190.
  10. Lin J-T, Chen P-C, Goud TV, Huang B-R, Lin T-C, Biellmann JF, Chen C-S* (2012) A Sulfhydryl-Reactive Ruthenium (II) Complex and its Conjugation to Protein G as a Universal Reagent for Fluorescent Immunoassays. PLoS ONE 7(4): e36086.
  11. Ho Y-H, Sung T-C, Chen C-S* (2012) Lactoferricin B inhibits the phosphorylation of two-component system response regulators: RasR and CreB. Molecular & Cellular Proteomics 11(4): 10.1074/mcp.M111.014720, 1-10.
  12. Horng H-C, Wen K-C, Su W-H, Chen C-S*, Wang P-H* (2012) Review of myomectomy. Taiwanese Journal of Obstetrics & Gynecology 51 (2012) 7-11.
  13. Du Y-H, Ho Y-H, Chuang Y-Z, Chen P-C, Chen C-S* (2011) Identification of Lactoferricin B Intracellular Targets Using an Escherichia coli Proteome Chip. PLoS ONE 6(12): e28197.
  14. Su W-H, Tsou T-S, Chen C-S, Ho T-Y, Lee W-L, Yu Y-Y, Chen T-H, Tan C-H, Wang P-H* (2011) Diagnosis of Chlamydia infection in women. Taiwan J Obstet Gynecol 50(3):261-7.
  15. Su W-H, Tsou T-S, Chen C-S, Ho T-Y, Lee W-L, Yu Y-Y, Chen T-H, Tan C-H, Wang P-H* (2011) Are we satisfied with the tools for the diagnosis of gonococcal infection in females? Journal of the Chinese Medical Association 74:430-434.
  16. Sandy T, Chen C-S, Zhu H, Escalante-Semerena1 JC* (2010) Nε-Lysine Acetylation of a Bacterial Transcription Factor Inhibits Its DNA-Binding Activity. PLoS ONE 5(12): e15123
  17. Su W-H, Cheng M-H, Lee W-L, Tsou T-S, Chang W-H, Chen C-S* Wang P-H* (2010) Nonsteroidal anti-inflammatory drugs for wounds: pain relief or excessive scar formation? Mediators of Inflammation 2010: doi:10.1155/2010/413238
  18. Chen M-S, Guo S-L, Cheng Y-C, Chen C-S* (2010) Comparison of Slide Surfaces for the Fabrication of Pain-Related Message Molecule Antibody Microarray. Chinese Journal of Pain 20:18-26.
  19. Chen C-S, Sullivan S, Anderson T, Tan AC, Alex PJ, Brant SR, Cuffari C, Bayless TM, Talor MV, Burek L, Wang H, Li R, Datta LW, Wu Y, Winslow RL*, Zhu H*, Li X* (2009) Identification of novel serological biomarkers for inflammatory bowel disease using E. coli proteome chip. Molecular & Cellular Proteomics 8:1765-1776.
  20. Chen C-S, Korobkova E, Chen H, Zhu J, Jian X, Tao S-C, He C*, Zhu H* (2008) A proteome chip approach reveals new DNA base damage recognition activities in Escherichia coli. Nature Methods 5: 69-74.
  21. Chen C-S, Baeumner AJ, Durst RA* (2008) Multiplexed immunoassays in food analysis. Handbook of Food Analysis Instruments Otles S (ed), CRC Press, Boca Raton, FL, p 439-454.
  22. Chen C-S, Tao S-C, Zhu H* (2008) Protein microarray technologies. Proteomics (Methods Express Series) O'Connor CD & Hames BD (eds), Scion Publishing Ltd., Bloxham, UK, p 183-205.
  23. Tao S-C, Chen C-S, Zhu H* (2007) Applications of protein microarray technology. Combinatorial Chemistry & High Throughput Screening 10(8): 706-718.
  24. Chen C-S, Zhu H* (2006) Protein microarrays. Biotechniques 40:423-429.
  25. Chen C-S, Durst RA* (2006) Simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes with an array-based immunosorbent assay using universal protein G-liposomal nanovesicles. Talanta 69: 232-238.
  26. Chen C-S, Yao J, Durst RA* (2006) Liposome encapsulation of fluorescent nanoparticles: quantum dots and silica nanoparticles. Journal of Nanoparticle Research 8:1033-1038. SCI 3.3
  27. Chen C-S (2006) Liposomal nanovesicles for multiplexed immunoassays: universal protein G-liposomes and fluorescent nanoparticle-liposomes. Ph.D dissertation.
  28. Chen C-S, Baeumner AJ, Durst RA* (2005) Protein G-liposomal nanovesicles as universal reagents for immunoassays. Talanta 67: 205-211.
  29. Chen C-S, Hsu C-K, Chiang B-H* (2002) Optimization of the enzymic process for manufacturing low-lactose milk containing oligosaccharides. Process Biochemistry 38: 801-808.
  30. Chen R-H* and Chen C-S (2000) Changes of polydispersity and limiting molecular weight of ultrasonic treated chitosan. Advances in Chitin Science 4:361-366.